Ownership Submission
FORM 4
Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).
UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP OF SECURITIES

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940
OMB APPROVAL
OMB Number: 3235-0287
Expires: January 31, 2005
Estimated average burden hours per response... 0.5

(Print or Type Responses)
1. Name and Address of Reporting Person *
RUED SCOTT D
  2. Issuer Name and Ticker or Trading Symbol
Roadrunner Transportation Systems, Inc. [RRTS]
5. Relationship of Reporting Person(s) to Issuer
(Check all applicable)
__X__ Director __X__ 10% Owner
__X__ Officer (give title below) _____ Other (specify below)
Chairman of the Board
(Last)
(First)
(Middle)
4900 S. PENNSYLVANIA AVENUE
3. Date of Earliest Transaction (Month/Day/Year)
05/18/2010
(Street)

CUDAHY, WI 53110
4. If Amendment, Date Original Filed(Month/Day/Year)
6. Individual or Joint/Group Filing(Check Applicable Line)
_X_ Form filed by One Reporting Person
___ Form filed by More than One Reporting Person
(City)
(State)
(Zip)
Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned
1.Title of Security
(Instr. 3)
2. Transaction Date (Month/Day/Year) 2A. Deemed Execution Date, if any (Month/Day/Year) 3. Transaction Code
(Instr. 8)
4. Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4 and 5)
5. Amount of Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 3 and 4)
6. Ownership Form: Direct (D) or Indirect (I)
(Instr. 4)
7. Nature of Indirect Beneficial Ownership
(Instr. 4)
Code V Amount (A) or (D) Price
Common Stock 05/18/2010   C   1,739,767 (1) A (2) 11,681,016 (3) I See Footnote (4) (5)
Common Stock 05/18/2010   C   2,565,609 (6) A (7) 14,246,625 (8) I See Footnote (4) (9)

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.
Persons who respond to the collection of information contained in this form are not required to respond unless the form displays a currently valid OMB control number. SEC 1474 (9-02)

Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned
(e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivative Security
(Instr. 3)
2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (Month/Day/Year) 3A. Deemed Execution Date, if any (Month/Day/Year) 4. Transaction Code
(Instr. 8)
5. Number of Derivative Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4, and 5)
6. Date Exercisable and Expiration Date
(Month/Day/Year)
7. Title and Amount of Underlying Securities
(Instr. 3 and 4)
8. Price of Derivative Security
(Instr. 5)
9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 4)
10. Ownership Form of Derivative Security: Direct (D) or Indirect (I)
(Instr. 4)
11. Nature of Indirect Beneficial Ownership
(Instr. 4)
Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares
Series B Convertible Preferred Stock (2) 05/18/2010   C     1,415,326 (10)   (11)   (11) Common Stock 1,739,767 (12) $ 0 0 I See Footnote (13) (14)

Reporting Owners

Reporting Owner Name / Address Relationships
 Director  10% Owner  Officer  Other
RUED SCOTT D
4900 S. PENNSYLVANIA AVENUE
CUDAHY, WI 53110
  X   X   Chairman of the Board  

Signatures

 Lisa M. Withers, Attorney-in-Fact   05/20/2010
**Signature of Reporting Person Date

Explanation of Responses:

* If the form is filed by more than one reporting person, see Instruction 4(b)(v).
** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
(1) Represents shares of common stock issued upon conversion of the Series B Preferred Stock (including accrued and unpaid dividends) in Table II. Represents 1,738,274 shares of common stock held by Thayer Equity Investors V, L.P. ("Thayer"); 554 shares of common stock held by TC Roadrunner-Dawes Holdings, L.L.C. ("TC Roadrunner"); and 939 shares of common stock held by TC Sargent Holdings, L.L.C. ("TC Sargent").
(2) The Series B Preferred Stock included in Table II (including all accrued and unpaid dividends) was converted into common stock immediately prior to the consummation of the Issuer's initial public offering for no additional consideration.
(3) Represents 11,632,192 shares of common stock held by Thayer; 24,369 shares of common stock held by TC Roadrunner; and 24,455 shares of common stock held by TC Sargent.
(4) The Reporting Person expressly disclaims beneficial ownership of the shares reported in Table I, except to the extent of his pecuniary interest therein. The filing of this form should not be deemed an admission that the Reporting Person is, for Section 16 purposes or otherwise, the beneficial owner of such shares.
(5) TC Equity Partners V, L.L.C. ("TC Equity") is the general partner of Thayer and Thayer | Hidden Creek Partners, L.L.C. ("Thayer | Hidden Creek") is the managing member of TC Equity. TC Co-Investors V, L.L.C. ("Co-Investors") is the managing member of each of TC Sargent and TC Roadrunner. Thayer | Hidden Creek Management, L.P. ("Management") is the sole manager of Co-Investors, and Thayer | Hidden Creek is the general partner of Management. As such, TC Equity, Co-Investors, Management, and Thayer | Hidden Creek may be deemed to be beneficial owners of the shares reported in Table I. Each of TC Equity, Co-Investors, Management, and Thayer | Hidden Creek expressly disclaims beneficial ownership of the shares reported in Table I except to the extent of its pecuniary interest therein. The filing of this form should not be deemed an admission that TC Equity, Co-Investors, Management, or Thayer | Hidden Creek is, for Section 16 purposes or otherwise, the beneficial owner of such shares.
(6) Represents 2,528,947 shares of common stock held by Thayer | Hidden Creek Partners II, L.P. ("Partners II") and 36,662 shares of common stock held by THC Co-Investors II, L.P. ("Co-Investors II") acquired in connection with the merger of a wholly owned subsidiary of the Issuer into Group Transportation Services Holdings, Inc. on May 18, 2010, as described in the Issuer's prospectus relating to its initial public offering.
(7) Acquired in connection with the merger of a wholly owned subsidiary of the Issuer into Group Transportation Services Holdings, Inc. on May 18, 2010, as described in the Issuer's prospectus relating to its initial public offering.
(8) Represents 11,632,192 shares of common stock held by Thayer; 24,369 shares of common stock held by TC Roadrunner; 24,455 shares of common stock held by TC Sargent; 2,528,947 shares held by Partners II, and 36,662 shares held by Co-Investors II.
(9) TC Equity is the general partner of Thayer and Thayer | Hidden Creek is the managing member of TC Equity. Co-Investors is the managing member of each of TC Sargent and TC Roadrunner. Management is the sole manager of Co-Investors, and Thayer | Hidden Creek is the general partner of Management. THCP Management II, L.P. ("THCP") is the general partner of Partners II and Co-Investors II, and Thayer | Hidden Creek is the general partner of THCP. As such, TC Equity, Co-Investors, Management, THCP, and Thayer | Hidden Creek may be deemed to be beneficial owners of the shares reported in Table I. Each of TC Equity, Co-Investors, Management, THCP, and Thayer | Hidden Creek expressly disclaims beneficial ownership of the shares except to the extent of its pecuniary interest therein. The filing of this form should not be deemed an admission that TC Equity, Co-Investors, Management, THCP, or Thayer | Hidden Creek is, for Section 16 purposes or otherwise, the beneficial owner of such shares.
(10) The Reporting Person previously reported 1,414,112 shares of common stock issuable upon conversion of Series B Convertible Preferred Stock (not including accrued and unpaid dividends) held by Thayer; 450 shares of common stock issuable upon conversion of Series B Convertible Preferred Stock (not including accrued and unpaid dividends) held by TC Roadrunner; and 764 shares of common stock issuable upon conversion of Series B Convertible Preferred Stock (not including accrued and unpaid dividends) held by TC Sargent.
(11) The Series B Convertible Preferred Stock was convertible at any time, at the Reporting Person's election, and had no expiration date.
(12) Immediately prior to the consummation of the Issuer's initial public offering, the Series B Preferred Stock (including accrued and unpaid dividends) was converted into common stock of the Issuer. Represents 1,738,274 shares of common stock held by Thayer; 554 shares of common stock held by TC Roadrunner; and 939 shares of common stock held by TC Sargent.
(13) TC Equity is the general partner of Thayer and Thayer | Hidden Creek is the managing member of TC Equity. Co-Investors is the managing member of each of TC Sargent and TC Roadrunner. Management is the sole manager of Co-Investors, and Thayer | Hidden Creek is the general partner of Management. As such TC Equity, Co-Investors, Management, and Thayer | Hidden Creek may be deemed to be beneficial owners of the shares reported in Table II. Each of TC Equity, Co-Investors, Management, and Thayer | Hidden Creek expressly disclaim beneficial ownership of the shares reported in Table II except to the extent of its pecuniary interest therein. The filing of this form should not be deemed an admission that TC Equity, Co-Investors, Management, or Thayer | Hidden Creek is, for Section 16 purposes or otherwise, the beneficial owner of such shares.
(14) The Reporting Person expressly disclaims beneficial ownership of the shares reported in Table II, except to the extent of his pecuniary interest therein. The filing of this form should not be deemed an admission that the Reporting Person is, for Section 16 purposes or otherwise, the beneficial owner of such shares.

Note: File three copies of this Form, one of which must be manually signed. If space is insufficient, see Instruction 6 for procedure.

Potential persons who are to respond to the collection of information contained in this form are not required to respond unless the form displays a currently valid OMB number.