UNITED STATES
                       SECURITIES AND EXCHANGE COMMISSION
                             Washington, D.C. 20549

                                    Form 13F
                               Form 13F COVER PAGE
Report for the Calendar Year or Quarter Ended: 3/31/2010
Check here if Amendment []; Amendment Number:
                                               -------
This Amendment (Check only one.):               [] is a restatement.
                                                [] adds new holdings entries.

Institutional Investment Manager Filing this Report:


Name:      Burke & Herbert Bank & Trust Co.

Address:   PO Box 268 Alexandria, VA 22314


Form 13F File Number:


The institutional investment manager filing this report and the person by whom
it is signed hereby represent that the person signing the report is authorized
to submit it, that all information contained herein is true, correct and
complete, and that it is understood that all required items, statements,
schedules, lists, and tables, are considered integral parts of this form.

Persons Signing this Report on Behalf of Reporting Manager:

Name:   Judith A. Cagnon
Title:  Vice President
Phone:  703-549-6600

Signature,  Place,  and  Date  of  Signing:

/s/ Judith A. Cagnon               Alexandria, VA                     4/6/2010
---------------------------------  ---------------------------------  ----------
[Signature]                        [City, State]                      [Date]



Report  Type  (Check  only  one):

[X]     13F HOLDINGS REPORT. (Check here if all holdings of this reporting
        manager are reported in this report.)

[ ]     13F NOTICE. (Check here if no holdings reported are in this report,
        and all holdings are reported by other reporting manager(s).)

[ ]     13F COMBINATION REPORT. (Check here if a portion of the holdings for
        this reporting manager are reported in this report and a portion are
        reported by other reporting manager(s).)

List of Other Managers Reporting for this Manager:




                              Form 13F SUMMARY PAGE

Report Summary:

Number of Other Included Managers:                    0

Form 13F Information Table Entry Total:             161

Form 13F Information Table Value Total:  $       86,984
                                         --------------
                                         (In Thousands)


List of Other Included Managers:

Provide a numbered list of the name(s) and 13F file number(s) of all
institutional investment managers with respect to which this report is filed,
other than the manager filing this report.
NONE







                                                  FORM 13F INFORMATION TABLE

          COLUMN 1              COLUMN 2    COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
---------------------------- -------------- --------- -------- ----------------- ---------- -------- -------------------
                                                       VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
       NAME OF ISSUER        TITLE OF CLASS   CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
---------------------------- -------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                   
TRANSOCEAN LTD               REG SHS        H8817H100       38      440 SH       SOLE                    440      0    0
TRANSOCEAN LTD               REG SHS        H8817H100      308    3,563 SH       DEFINED               3,563      0    0
AT&T INC                     COM            00206R102      738   28,548 SH       SOLE                 28,548      0    0
AT&T INC                     COM            00206R102      725   28,061 SH       DEFINED              28,061      0    0
ABBOTT LABS                  COM            002824100      611   11,600 SH       SOLE                 11,600      0    0
ABBOTT LABS                  COM            002824100      606   11,510 SH       DEFINED              11,510      0    0
ALLIED CAP CORP NEW          COM            01903Q108        0       25 SH       SOLE                     25      0    0
ALLIED CAP CORP NEW          COM            01903Q108       79   15,975 SH       DEFINED              15,975      0    0
ALTRIA GROUP INC             COM            02209S103      404   19,700 SH       SOLE                 19,700      0    0
ALTRIA GROUP INC             COM            02209S103      162    7,900 SH       DEFINED               7,900      0    0
AMERICAN EXPRESS CO          COM            025816109      205    4,966 SH       SOLE                  4,966      0    0
AMERICAN EXPRESS CO          COM            025816109       31      750 SH       DEFINED                 750      0    0
APPLE INC                    COM            037833100      186      790 SH       SOLE                    790      0    0
APPLE INC                    COM            037833100      400    1,700 SH       DEFINED               1,700      0    0
AUTOMATIC DATA PROCESSING IN COM            053015103       13      300 SH       SOLE                    300      0    0
AUTOMATIC DATA PROCESSING IN COM            053015103      294    6,600 SH       DEFINED               6,600      0    0
BP PLC                       SPON ADR       055622104      818   14,332 SH       SOLE                 14,332      0    0
BP PLC                       SPON ADR       055622104      291    5,097 SH       DEFINED               5,097      0    0
BANCO BILBAO VIZCAYA ARGENTA SPON ADR       05946K101      192   14,045 SH       SOLE                 14,045      0    0
BANK OF AMERICA CORPORATION  COM            060505104      138    7,758 SH       SOLE                  7,758      0    0
BANK OF AMERICA CORPORATION  COM            060505104      714   40,008 SH       DEFINED              40,008      0    0
BANK OF NEW YORK MELLON CORP COM            064058100        5      150 SH       SOLE                    150      0    0
BANK OF NEW YORK MELLON CORP COM            064058100      560   18,139 SH       DEFINED              18,139      0    0
BAXTER INTL INC              COM            071813109      189    3,250 SH       SOLE                  3,250      0    0
BAXTER INTL INC              COM            071813109       44      750 SH       DEFINED                 750      0    0
BERKSHIRE HATHAWAY INC DEL   CL A           084670108      365        3 SH       SOLE                      3      0    0
BERKSHIRE HATHAWAY INC DEL   CL A           084670108      244        2 SH       DEFINED                   2      0    0
BLACKROCK INC                COM            09247X101       93      425 SH       SOLE                    425      0    0
BLACKROCK INC                COM            09247X101      185      850 SH       DEFINED                 850      0    0
BOEING CO                    COM            097023105      203    2,802 SH       SOLE                  2,802      0    0
BOEING CO                    COM            097023105       36      500 SH       DEFINED                 500      0    0
BRISTOL MYERS SQUIBB CO      COM            110122108      265    9,926 SH       SOLE                  9,926      0    0
BRISTOL MYERS SQUIBB CO      COM            110122108      161    6,020 SH       DEFINED               6,020      0    0
BURKE&HERBERT B&T ALEXANDRIA COM            121331102   12,677    6,672 SH       SOLE                  6,672      0    0
BURKE&HERBERT B&T ALEXANDRIA COM            121331102    1,556      819 SH       DEFINED                 819      0    0
CIGNA CORP                   COM            125509109      555   15,180 SH       DEFINED              15,180      0    0
CVS CAREMARK CORPORATION     COM            126650100      320    8,750 SH       DEFINED               8,750      0    0
CHEVRON CORP NEW             COM            166764100      815   10,749 SH       SOLE                 10,749      0    0
CHEVRON CORP NEW             COM            166764100    1,704   22,471 SH       DEFINED              22,471      0    0
CISCO SYS INC                COM            17275R102      365   14,024 SH       SOLE                 14,024      0    0
CISCO SYS INC                COM            17275R102      206    7,900 SH       DEFINED               7,900      0    0
CITIGROUP INC                COM            172967101       12    2,903 SH       SOLE                  2,903      0    0
CITIGROUP INC                COM            172967101      384   94,695 SH       DEFINED              94,695      0    0
COCA COLA CO                 COM            191216100      648   11,780 SH       SOLE                 11,780      0    0
COCA COLA CO                 COM            191216100      580   10,550 SH       DEFINED              10,550      0    0
COLGATE PALMOLIVE CO         COM            194162103      281    3,295 SH       SOLE                  3,295      0    0
COLGATE PALMOLIVE CO         COM            194162103      166    1,950 SH       DEFINED               1,950      0    0
CONOCOPHILLIPS               COM            20825C104       30      580 SH       SOLE                    580      0    0
CONOCOPHILLIPS               COM            20825C104      224    4,380 SH       DEFINED               4,380      0    0
DELL INC                     COM            24702R101       65    4,350 SH       SOLE                  4,350      0    0
DELL INC                     COM            24702R101      146    9,700 SH       DEFINED               9,700      0    0
DISNEY WALT CO               COM            254687106      265    7,592 SH       SOLE                  7,592      0    0
DISNEY WALT CO               COM            254687106      475   13,600 SH       DEFINED              13,600      0    0
DOMINION RES INC VA NEW      COM            25746U109      446   10,842 SH       SOLE                 10,842      0    0
DOMINION RES INC VA NEW      COM            25746U109      416   10,124 SH       DEFINED              10,124      0    0
DU PONT E I DE NEMOURS & CO  COM            263534109      479   12,872 SH       SOLE                 12,872      0    0
DU PONT E I DE NEMOURS & CO  COM            263534109      679   18,226 SH       DEFINED              18,226      0    0
DUKE ENERGY CORP NEW         COM            26441C105       29    1,763 SH       SOLE                  1,763      0    0
DUKE ENERGY CORP NEW         COM            26441C105      175   10,700 SH       DEFINED              10,700      0    0
DUN & BRADSTREET CORP DEL NE COM            26483E100      238    3,200 SH       DEFINED               3,200      0    0






                                                  FORM 13F INFORMATION TABLE

          COLUMN 1              COLUMN 2    COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
---------------------------- -------------- --------- -------- ----------------- ---------- -------- -------------------
                                                       VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
       NAME OF ISSUER        TITLE OF CLASS   CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
---------------------------- -------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                   
EMERSON ELEC CO              COM            291011104      169    3,364 SH       SOLE                  3,364      0    0
EMERSON ELEC CO              COM            291011104       94    1,860 SH       DEFINED               1,860      0    0
EXELON CORP                  COM            30161N101      126    2,882 SH       SOLE                  2,882      0    0
EXELON CORP                  COM            30161N101      166    3,800 SH       DEFINED               3,800      0    0
EXXON MOBIL CORP             COM            30231G102    2,915   43,518 SH       SOLE                 43,518      0    0
EXXON MOBIL CORP             COM            30231G102    4,459   66,574 SH       DEFINED              66,574      0    0
FPL GROUP INC                COM            302571104      243    5,032 SH       SOLE                  5,032      0    0
FPL GROUP INC                COM            302571104      152    3,140 SH       DEFINED               3,140      0    0
FEDERAL REALTY INVT TR       SH BEN INT NEW 313747206      666    9,150 SH       DEFINED               9,150      0    0
FLUOR CORP NEW               COM            343412102      293    6,300 SH       DEFINED               6,300      0    0
FORTUNE BRANDS INC           COM            349631101      364    7,500 SH       DEFINED               7,500      0    0
FREEPORT-MCMORAN COPPER & GO COM            35671D857      937   11,221 SH       DEFINED              11,221      0    0
GENERAL DYNAMICS CORP        COM            369550108       77    1,000 SH       SOLE                  1,000      0    0
GENERAL DYNAMICS CORP        COM            369550108      147    1,900 SH       DEFINED               1,900      0    0
GENERAL ELECTRIC CO          COM            369604103    1,903  104,541 SH       SOLE                104,541      0    0
GENERAL ELECTRIC CO          COM            369604103    2,198  120,773 SH       DEFINED             120,773      0    0
GOLDMAN SACHS GROUP INC      COM            38141G104       66      385 SH       SOLE                    385      0    0
GOLDMAN SACHS GROUP INC      COM            38141G104      137      805 SH       DEFINED                 805      0    0
GOOGLE INC                   CL A           38259P508       17       30 SH       SOLE                     30      0    0
GOOGLE INC                   CL A           38259P508      193      340 SH       DEFINED                 340      0    0
HARRIS CORP DEL              COM            413875105      104    2,200 SH       SOLE                  2,200      0    0
HARRIS CORP DEL              COM            413875105      119    2,500 SH       DEFINED               2,500      0    0
HEWLETT PACKARD CO           COM            428236103    1,106   20,812 SH       SOLE                 20,812      0    0
HEWLETT PACKARD CO           COM            428236103    1,327   24,975 SH       DEFINED              24,975      0    0
HOME DEPOT INC               COM            437076102      154    4,775 SH       SOLE                  4,775      0    0
HOME DEPOT INC               COM            437076102      184    5,683 SH       DEFINED               5,683      0    0
HONEYWELL INTL INC           COM            438516106      238    5,262 SH       SOLE                  5,262      0    0
HONEYWELL INTL INC           COM            438516106      267    5,900 SH       DEFINED               5,900      0    0
INTEL CORP                   COM            458140100      374   16,778 SH       SOLE                 16,778      0    0
INTEL CORP                   COM            458140100      280   12,575 SH       DEFINED              12,575      0    0
INTERNATIONAL BUSINESS MACHS COM            459200101      694    5,412 SH       SOLE                  5,412      0    0
INTERNATIONAL BUSINESS MACHS COM            459200101      683    5,327 SH       DEFINED               5,327      0    0
ISHARES TR INDEX             BARCLY USAGG B 464287226      246    2,365 SH       SOLE                  2,365      0    0
ISHARES TR INDEX             RUSSELL 3000   464287689      225    3,270 SH       DEFINED               3,270      0    0
JPMORGAN CHASE & CO          COM            46625H100      197    4,405 SH       SOLE                  4,405      0    0
JPMORGAN CHASE & CO          COM            46625H100      111    2,470 SH       DEFINED               2,470      0    0
JOHNSON & JOHNSON            COM            478160104    3,054   46,842 SH       SOLE                 46,842      0    0
JOHNSON & JOHNSON            COM            478160104      604    9,260 SH       DEFINED               9,260      0    0
KIMBERLY CLARK CORP          COM            494368103      281    4,469 SH       SOLE                  4,469      0    0
KIMBERLY CLARK CORP          COM            494368103      182    2,898 SH       DEFINED               2,498    400    0
KRAFT FOODS INC              CL A           50075N104      397   13,117 SH       SOLE                 13,117      0    0
KRAFT FOODS INC              CL A           50075N104       59    1,961 SH       DEFINED               1,961      0    0
LOCKHEED MARTIN CORP         COM            539830109      190    2,280 SH       SOLE                  2,280      0    0
LOCKHEED MARTIN CORP         COM            539830109       50      600 SH       DEFINED                 600      0    0
LOWES COS INC                COM            548661107       72    2,950 SH       SOLE                  2,950      0    0
LOWES COS INC                COM            548661107      355   14,650 SH       DEFINED              14,650      0    0
MANULIFE FINL CORP           COM            56501R106      197    9,988 SH       SOLE                  9,988      0    0
MANULIFE FINL CORP           COM            56501R106      425   21,608 SH       DEFINED              21,608      0    0
MCDONALDS CORP               COM            580135101      217    3,254 SH       SOLE                  3,254      0    0
MCDONALDS CORP               COM            580135101       97    1,450 SH       DEFINED               1,450      0    0
MEDCO HEALTH SOLUTIONS INC   COM            58405U102      314    4,870 SH       SOLE                  4,870      0    0
MEDCO HEALTH SOLUTIONS INC   COM            58405U102       92    1,422 SH       DEFINED               1,422      0    0
MEDTRONIC INC                COM            585055106      318    7,054 SH       SOLE                  7,054      0    0
MEDTRONIC INC                COM            585055106      135    3,000 SH       DEFINED               3,000      0    0
MERCK & CO INC NEW           COM            58933Y105      573   15,330 SH       SOLE                 15,330      0    0
MERCK & CO INC NEW           COM            58933Y105      707   18,930 SH       DEFINED              18,930      0    0
MICROSOFT CORP               COM            594918104      361   12,329 SH       SOLE                 12,329      0    0
MICROSOFT CORP               COM            594918104      910   31,075 SH       DEFINED              31,075      0    0
NORFOLK SOUTHERN CORP        COM            655844108      747   13,369 SH       SOLE                 13,369      0    0
NORFOLK SOUTHERN CORP        COM            655844108      402    7,200 SH       DEFINED               7,200      0    0






                                                  FORM 13F INFORMATION TABLE

          COLUMN 1              COLUMN 2    COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
---------------------------- -------------- --------- -------- ----------------- ---------- -------- -------------------
                                                       VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
       NAME OF ISSUER        TITLE OF CLASS   CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
---------------------------- -------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                   
ORACLE CORP                  COM            68389X105       84    3,250 SH       SOLE                  3,250      0    0
ORACLE CORP                  COM            68389X105      433   16,830 SH       DEFINED              16,830      0    0
PEPSICO INC                  COM            713448108      640    9,675 SH       SOLE                  9,675      0    0
PEPSICO INC                  COM            713448108    1,024   15,473 SH       DEFINED              15,473      0    0
PFIZER INC                   COM            717081103    1,031   60,114 SH       SOLE                 60,114      0    0
PFIZER INC                   COM            717081103    1,373   80,039 SH       DEFINED              80,039      0    0
PHILIP MORRIS INTL INC       COM            718172109    1,017   19,500 SH       SOLE                 19,500      0    0
PHILIP MORRIS INTL INC       COM            718172109      383    7,342 SH       DEFINED               7,342      0    0
PROCTER & GAMBLE CO          COM            742718109      630    9,950 SH       SOLE                  9,950      0    0
PROCTER & GAMBLE CO          COM            742718109      445    7,041 SH       DEFINED               7,041      0    0
PROTECTIVE LIFE CORP         COM            743674103    5,112  232,488 SH       SOLE                232,488      0    0
QWEST COMMUNICATIONS INTL IN COM            749121109        2      361 SH       SOLE                    361      0    0
QWEST COMMUNICATIONS INTL IN COM            749121109       60   11,550 SH       DEFINED              11,550      0    0
REGIONS FINANCIAL CORP NEW   COM            7591EP100      637   81,091 SH       SOLE                 81,091      0    0
REGIONS FINANCIAL CORP NEW   COM            7591EP100       21    2,710 SH       DEFINED               2,710      0    0
ROYAL DUTCH SHELL PLC        SPONS ADR A    780259206      311    5,370 SH       SOLE                  5,370      0    0
SARA LEE CORP                COM            803111103      117    8,385 SH       SOLE                  8,385      0    0
SARA LEE CORP                COM            803111103      116    8,300 SH       DEFINED               8,300      0    0
SCHLUMBERGER LTD             COM            806857108      241    3,793 SH       SOLE                  3,793      0    0
SCHLUMBERGER LTD             COM            806857108      501    7,890 SH       DEFINED               7,890      0    0
SPACE PROPULSION SYS INC     COM            846192102        1   16,250 SH       SOLE                 16,250      0    0
TJX COS INC NEW              COM            872540109      221    5,200 SH       DEFINED               5,200      0    0
TARGET CORP                  COM            87612E106       70    1,326 SH       SOLE                  1,326      0    0
TARGET CORP                  COM            87612E106      274    5,210 SH       DEFINED               5,210      0    0
TEXAS INSTRS INC             COM            882508104      131    5,342 SH       SOLE                  5,342      0    0
TEXAS INSTRS INC             COM            882508104      186    7,607 SH       DEFINED               7,607      0    0
3M CO                        COM            88579Y101      569    6,813 SH       SOLE                  6,813      0    0
3M CO                        COM            88579Y101      281    3,358 SH       DEFINED               3,358      0    0
TRAVELERS COMPANIES INC      COM            89417E109        2       45 SH       SOLE                     45      0    0
TRAVELERS COMPANIES INC      COM            89417E109      238    4,421 SH       DEFINED               4,421      0    0
UNITED TECHNOLOGIES CORP     COM            913017109      245    3,330 SH       SOLE                  3,330      0    0
UNITED TECHNOLOGIES CORP     COM            913017109      674    9,155 SH       DEFINED               9,155      0    0
UNITEDHEALTH GROUP INC       COM            91324P102      186    5,700 SH       SOLE                  5,700      0    0
UNITEDHEALTH GROUP INC       COM            91324P102       26      800 SH       DEFINED                 800      0    0
VERIZON COMMUNICATIONS INC   COM            92343V104      637   20,551 SH       SOLE                 20,551      0    0
VERIZON COMMUNICATIONS INC   COM            92343V104      906   29,215 SH       DEFINED              29,215      0    0
WAL MART STORES INC          COM            931142103      981   17,636 SH       SOLE                 17,636      0    0
WAL MART STORES INC          COM            931142103      520    9,350 SH       DEFINED               9,350      0    0
WELLPOINT INC                COM            94973V107      348    5,400 SH       DEFINED               5,400      0    0
WELLS FARGO & CO NEW         COM            949746101      454   14,576 SH       SOLE                 14,576      0    0
WELLS FARGO & CO NEW         COM            949746101      247    7,928 SH       DEFINED               7,928      0    0